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2.4K Views. University of Wisconsin. This protocol can be used to investigate whether volatile anesthetics disrupts some synopsis more than others, to determine if their effects are cell type or as pathway specific, and to determine what effects occur at the population level. The main advantage of this technique is the specificity with which we can assess the effects of volatile anesthetics. We can target specific pathways and cell types in simultaneously assay population activity.Begin by preparing the tissue block for s...